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TRF reverses age-related fibrosis in adipose tissue. (A) Representative images of Masson’s Trichrome staining for collagen in adipose sections from young, aged, and aged-TRF animals. Black arrows point to blue-stained collagen fibers in adipose sections. (B) Image analysis from Trichrome staining showing % collagen-stained area in adipose sections. n = 5-6/group. (C) Semiquantitative analysis of collagen levels using <t>Sirius</t> <t>Red/Fast</t> Green staining after normalization to total protein levels. * p < .05, ** p < .01, *** p < .005, and **** p < .001 between the indicated groups. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue; TRF, time-restricted feeding.
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TRF reverses age-related fibrosis in adipose tissue. (A) Representative images of Masson’s Trichrome staining for collagen in adipose sections from young, aged, and aged-TRF animals. Black arrows point to blue-stained collagen fibers in adipose sections. (B) Image analysis from Trichrome staining showing % collagen-stained area in adipose sections. n = 5-6/group. (C) Semiquantitative analysis of collagen levels using Sirius Red/Fast Green staining after normalization to total protein levels. * p < .05, ** p < .01, *** p < .005, and **** p < .001 between the indicated groups. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue; TRF, time-restricted feeding.

Journal: The Journals of Gerontology Series A: Biological Sciences and Medical Sciences

Article Title: Time-restricted feeding improves metabolic flexibility, promotes beiging, and mitigates fibro-inflammation in the adipose tissue of aged mice

doi: 10.1093/gerona/glag085

Figure Lengend Snippet: TRF reverses age-related fibrosis in adipose tissue. (A) Representative images of Masson’s Trichrome staining for collagen in adipose sections from young, aged, and aged-TRF animals. Black arrows point to blue-stained collagen fibers in adipose sections. (B) Image analysis from Trichrome staining showing % collagen-stained area in adipose sections. n = 5-6/group. (C) Semiquantitative analysis of collagen levels using Sirius Red/Fast Green staining after normalization to total protein levels. * p < .05, ** p < .01, *** p < .005, and **** p < .001 between the indicated groups. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue; TRF, time-restricted feeding.

Article Snippet: The semiquantitative collagen detection kit for total tissue (Sirius Red/Fast Green collagen staining kit, Chondrex, Inc., catalog # 9046, Woodinville, WA) was used to assess the total amount of collagen and noncollagenous proteins in adipose tissue sections from the three experimental groups.

Techniques: Staining